ABSTRACT
OBJECTIVE: Identify the influence of ovarian hormone deprivation in expression genes on the lower urinary tract of rats. MATERIALS AND METHODS: This study deals with gene screening on lower urinary tract of rats. Fifty isogenic rats divided in two groups of twenty-five animals have their lower urinary tract surgically removed: group I, ovariectomized rats 30 days prior to surgery; group II, non-ovariectomized rats. Total RNA was isolated from bladder and urethra, and differential expression of genes was analyzed quantitative, qualitative and comparatively by array technology and RT-PCR. RESULTS: A total of 76 candidate genes were identified as differentially expressed between the groups, 26 being lower expressed in group II, and 50 in group I. Among them, differential expression validation was confirmed by RT-PCR for three lower expressed genes in group I: Vascular Endothelial Growth Factor (VEGF), Beta-2 Microglobulin (B2M) and Cytochrome c Oxidase subunit I (COX I). CONCLUSION: Ovarian hormone deprivation influences the expression genes on lower urinary tract. We demonstrated that a 30-day period of castration down regulate the expression of VEGF, B2M and COX I in adult rats which are involved in activities of angiogenesis, immune responses and cellular metabolism respectively.
Subject(s)
Animals , Female , Rats , Estrogens/deficiency , Gene Expression , Urinary Tract/metabolism , Urologic Diseases/genetics , Vascular Endothelial Growth Factor A/genetics , Cyclooxygenase 1/genetics , Disease Models, Animal , Estrogens/pharmacology , Gene Expression Profiling , In Situ Hybridization , Membrane Proteins/genetics , Oligonucleotide Array Sequence Analysis , Ovariectomy , Ovary/physiology , Rats, Wistar , Reverse Transcriptase Polymerase Chain Reaction , Urinary Tract/drug effects , Urologic Diseases/metabolism , Vascular Endothelial Growth Factor A/blood , /geneticsABSTRACT
A ocorrência de infecção urinária na gestação continua sendo importante fator de morbidade, principalmente quando não há suspeita de bacteriúria assintomática. O rastreamento sistemático com exame qualitativo de urina, urocultura e teste de sensibilidade aos antibióticos, em todas as gestantes, tornam-se economicamente inviáveis na medicina previdenciária. É fundamental adotar critérios de risco para identificar aquelas para as quais se deve pedir exames. Frente à multiplicidade de novos antibióticos, o tratamento deve ser criteriosamente elaborado. Os esquemas terapêuticos tradicionais, há muito utilizados, continuam válidos. Com esta política preservam-se antibióticos mais novos para eventos mais sérios. Não diagnosticar infecção urinária numa gestante continua sendo o principal fator para ocorrência de infecções complicadas como a pielonefrite